We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Refer to safety data sheet for handling instructions. Citations are publications that use tocris products. Identification of the substancemixture and of the companyundertaking product identifier product code 461120 product name blasticidin, powder chemical name not applicable reach registration numberno registration number is given yet for this substance substances in this mixture. Blasticidin s hydrochloride is a salt of blasticidin s, an amphoteric nucleoside produced by several species of streptomyces, first reported in the late. If the products does not requires msds, please certify the products are nonhazardous products for which no msds is. Blasticidin s hcl is a nucleoside antibiotic isolated from stretomyces girseochromogenes, and acts as a dna and protein synthesis inhibitor, used to select transfected cells carrying bsr or bsd resistance genes. Blasticidin s hydrochloride, like puromycin sc205821, belongs to a group of cytosine amino nucleoside antibiotics. At goldbio, we believe that putting our quality products in capable hands leads to discoveries that impact the entire world.
Ag scientifics blasticidin s hcl solution is a sterile filterate solution. Blasticidin s hcl is an aminoacyl nucleoside antibiotic isolated from streptomyces griseochromogenes. It has been reported to interfere with translocation 1 and to cause mistranslation at the 70s ribosome 2. It inhibits protein synthesis in bacteria and eukaryotes. The isolated mrna is suitable for use in all downstream applications. Blasticidin s is a peptidyl nuncleoside antibiotic isolated from the culture broth ofstretomyces girseochromogenes. Blasticidin s is a nucleoside antibiotic isolated from streptomyces.
Australian msds statementclassified according to the criteria of worksafe australia. Blasticidin s hcl is a nucleoside antibiotic isolated from streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. Hygromycin b is an aminoglycoside antibiotic produced by streptomyces hygroscopicus. Honeywell fluka inorganic chemicals and reagents are suitable for a wide variety of analytical applications, including leading brands like hydranal reagents for karl fischer titrations and traceselect highpurity inorganics for measuring trace and lowmetal content, plus a full offering of acids, bases. Blasticidin, from streptomyces griseochromogenes, is an antibiotic commonly used in gene selection. Transfection of cells with the bsr gene confers resistance to blasticidin s cas 2079007, 3539. However, we make no warranty of merchantability or any other warranty, express or implied, w ith respect to such information, and we assume no liability resulting from its use. It also has fungicidal properties and prevents rice blast disease. The aqueous solution of the commercially available n4benzylamino benzenesulfonic acid babs salt of the antibiotic has a ph of 6. Blasticidin s hcl solution is a nucleoside antibiotic supplied at 10 mgml in hepes buffer for use as a selection. In mouse leukemia l5178y cells, blasticidin s inhibited protein synthesis and caused the degradation of presumptive mrna 1. Notably, the mean nucleosome movement for 30 ms 51 nm in the xy plane in interphase chromatin. Blasticidin s hcl is an antibiotic inhibiting protein synthesis.
The cards are data sheets intended to provide essential safety and health information on chemicals in a clear and concise way. Benzenesulfonic acid an overview sciencedirect topics. The dynabeads mrna direct kit is designed for simple and rapid isolation of pure, intact polyadenylated polya mrna directly from the crude lysate of animal and plant cells and tissues. Blasticidin s hydrochloride was originally isolated by acetone precipitation.
B4402 blasticidin s hydrochloride references yoshinari t, sakuda s, watanabe m, et al. Blasticidin s is a peptidyl nucleaside antibiotic isolated from the culture broth of streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryotes. Please use the form below to provide feedback related to the content on this product. Blasticidin is a selection antibiotic for mammalian and bacterial cells. Blasticidin s hcl supplier cas 3539 tocris bioscience. Blasticidin s hcl catalog numbers a11902 and a11903 doc. Blasticidin s hcl is an antibiotic that demonstrates a strong inhibitory effect on protein synthesis in eukaryotic and prokaryotic cells. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Yk4279 is a potent inhibitor of ewsfli1 binding to rna helicase a rha.
Blasticidin s is isolated from streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells, it acts quickly by inhibiting peptide bond formation by the ribosome. Hop humulus lupulus zeatin mixed isomers for tissue culture. Since the informations vary, it is recommended to determine the. Blasticidin s hcl blasticidin s hcl is a nucleoside antibiotic isolated from stretomyces girseochromogenes, and acts as a dna and protein synthesis inhibitor, used to select transfected cells carrying bsr or bsd resistance genes. New metabolic pathway for converting blasticidin s in aspergillus flavus and. Bear1,2,3 both authors contributed equally to this work. Nbds and the loops extending from the msds in coupling the catalytic domains to the gating of the anion permeation pathway 16, 17. Hygromycin b is used as a selective agent in molecular genetics. Looking for rpi blasticidin s hydrochloride solution, 100mg liquid 30tx31. Our products cover applications in chemistry, biological research, pharma, healthcare and production.
Blasticidin is active against both prokaryotic and eukaryotic. Blasticidin s is isolated from streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Blasticidin s acts quickly by inhibiting peptide bond formation by the ribosome. The information provided on this material safety data sheet is furnished in good faith and without warranty of any kind. Details of the supplier of the safety data sheet company. Blasticidin s is used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or bsd. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy.
Cas 2079007 blasticidin s hydrochloride, 150477 blasticidin s hydrochloride msds toxicity property. Gold biotechnology is about putting our quality products into capable hands to leads to discoveries that impact the entire world. The above information is believed to be correct and should be used only as a guide. Chemical npfa codes free ebook download as excel spreadsheet. Product name blasticidin s hcl chemical name synonyms lkt laboratories, inc 545 phalen blvd. Discontinued some products of fujifilm cellnest series. Supplied as a 10mgml sterile solution in hepes buffer. It works on eukaryotic and prokaryotic cells, and is used to study protein synthesis. Blasticidin s hcl stock solution mammalian 210 mgml. Improve lentiviral production using lipofectamine 3000.
Not intended for human or animal diagnostic or therapeutic uses. Blasticidin s inhibits protein synthesis by inducing deformations in psite trna. Blasticidin s hydrochloride is a salt of blasticidin s, an amphoteric nucleoside produced by sever al species of streptomyces, first reported in the late 1950s. Blasticidin s hcl stock solution mammalian210 mgml 1. The singlenucleosome imaging in the present study demonstrated such local nucleosome dynamics. Section 1 chemical product and company identification product name hydrochloric acid conc. Resistance is conferred by expression of either one of two blasticidin s deaminase genes. Blasticidin s hydrochloride safety data sheet section 2. Programme in molecular structure and function in the research institute1, hospital for sick children and the departments of physiology and biochemistry2 and the institute of medical. Blasticidin s hcl solution cas 3539 scbt santa cruz. More recently, blasticidin s has been used as a marker for strain manipulations. Probing conformational rescue induced by a chemical. The main target users are workers and those responsible for occupational safety and health.
Blasticidine s hydrochloride blasticidin s sigmaaldrich. Self contained breathing apparatus or nioshmsha approved respirator is recommended. Blasticidin s hcl solution is a nucleoside antibiotic supplied at 10 mgml in hepes buffer for use as a selection antibiotic cited in 5 publications. It functions by inhibiting peptide bond formation at the ribosome in both eukaryotic and prokaryotic cells. Blasticidin s inhibits aflatoxin production in species of aspergillus. Download pdf version invitrogen lipofectamine 3000 transfection reagent is a highly efficient, costeffective tool for lentiviral production figure 1. Blasticidin s hcl solution is supplied ready to use at 10 mgml in hepes buffer and sterile filtered.
Do you know of a great paper that uses blasticidin s hcl from tocris. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Cystic fibrosis cf is caused by mutations in the cystic fibrosis transmembrane conductance regulator cftr gene that cause loss of function of the cftr channel on the apical surface of epithelial cells. Material may be irritating to the mucous membranes and upper respiratory tract. Our success relies on our commitment to the success of those. The blasticidin s resistance gene bsr, found in bacillus cereus k55s1. The working concentration depends on the organism choosen. Blasticidin s is an antibiotic that also exhibits antiparasitic activity. Blasticidin s inhibits translation by trapping deformed trna on the ribosome. Blasticidin s is an antifungal agent with particularly potent activity against the rice pathogen, piricularia oryzae, for which it was used commercially for some time in japan. Blasticidin s inhibits protein synthesis and is active against bacteria, tumour cell lines and nematodes.
This versatile reagent enables high viral titers even with genes that are large or difficult to package. We are manufacturers of laboratory reagents and chemicals for industrial use. Search results for blasticidin s hcl, powder at sigmaaldrich. Blasticidin causes trna to bond more strongly to the p site of the large ribosomal subunit while simultaneously deforming the trna, thus inhibiting proper translation. Blasticidinsensitive cells should be killed at all concentrations tested 2. Our success relies on our commitment to the success of those whom we supply.
Blasticidin s is a nucleoside antibiotic isolated from streptomyces griseochromogenes. Find msds or sds, a coa, data sheets and more information. Hydrochloric acid hydrogen chloride hydrochloric acid. It is highly efficient for selecting eukaryotic cells trans. Blasticidin s hcl, powder thermo fisher scientific. Our msds database is an easy to use, do it yourself tool, and best of all. Blasticidin s hcl 10 mgml thermo fisher scientific. Blasticidin s hcl r21001 50mg please let us have best alternate products for the above at your earliest. Carbon oxides, nitrogen oxides, hydrogen chloride 4. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Coa, qc data and msds documents are available in one week after order is received. Currently there are no citations for blasticidin s hcl. Shop online for a wide selection of gibco blasticidin s hcl 10 mgml selective antibiotic in concentration range of 50g100gml.
The primary aim of the cards is to promote the safe use of chemicals in the workplace. The icsc project is a common undertaking between the world health organization who and. Blasticidin s belongs to the aminoacylnucleoside class of antibiotics. Blasticidin s hydrochloride purity 98%, is in stock.
Blasticidin s hcl is a nucleoside antibiotic isolated from streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells takeuchi et al. Blasticidin is an effective selective agent for transformed cells. Bsd from aspergillus terreus or bsr from bacillus cereus. Blasticidin s is used to select transfected cells carrying bsr or bsd resistance genes. The ph of the aqueous solution of the free base is 9. Relevant identified uses of the substance or mixture and uses advised against identified uses laboratory reagent. Blasticidin s is a peptidyl nucleoside antibiotic isolated from the culture. Resistance to blasticidin is conferred by the blasticidin resistance gene from bacillus cereus bsr. Read the safety data sheets sdss and follow the handling. Blasticidin s hcl is lot qualified by performing a kill curve on blasticidinsensitive and blasticidin resistant mammalian cell lines. The major cfcausing mutation, f508delcftr, is misfolded, retained in the endoplasmic reticulum, and degraded. For historical reasons only the salt has been routinely available to researchers.
Identification of the substancemixture and of the companyundertaking. Blasticidin s is a nucleoside produced by several species of streptomyces, first reported in the late 1950s. New metabolic pathway for converting blasticidin s in aspergillus flavus and inhibitory activity of aflatoxin production by blasticidin s metabolites. No bla5x, bla1x relevant identified uses of the substance or mixture and uses advised against for research use only. Current shipping out time is about 2 weeks after order is received. Advance your analysis with fluka analytical standards every standard we sell comes complete with a very detailed printed certificate of analysis coa and is eligible for free expert technical support ensuring you have the information you need to quickly start your analysis, and pass relevant audits. It is sterile, cell culture tested, and available as a solution or powder.
Rpi blasticidin s hydrochloride solution, 100mg liquid. Blasticidin s is a nucleoside antibiotic isolated from streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin s hydrochloride is soluble in water or buffers e. Blasticidin s, hcl no data available no data available no data available principle routes of exposure potential health effects eyes.
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